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KMID : 1094720160210010128
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Biotechnology and Bioprocess Engineering
2016 Volume.21 No. 1 p.128 ~ p.133
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A rapidly new-typed detection of norovirus based on F0F1-ATPase molecular motor biosensor
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Zhao Zhuo
Zhang Jie
Xu Mei-Ling
Liu Zhi-Peng
Wang Hua
Liu Ming
Yu Yan-Yan
Sun Li
Zhang Hui
Wu Hai-Yan
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Abstract
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In order to adapt port rapid detection of food borne norovirus, presently we developed a new typed detection method based on F0F1-ATPase molecular motor biosensor. A specific probe was encompassed the conservative region of norovirus and F0F1-ATPase within chromatophore was constructed as a molecular motor biosensor through the “ε-subunit antibody-streptomycin-biotin-probe” system. Norovirus was captured based on probe-RNA specific binding. Our results demonstrated that the Limit of Quantification (LOQ) is 0.005 ng/mL for NV RNA and also demonstrated that this method possesses specificity and none cross-reaction for food borne virus. What’s more, the experiment used this method could be accomplished in 1 h. We detected 10 samples by using this method and the results were consistent with RT-PCR results. Overall, based on F0F1-ATPase molecular motors biosensor system we firstly established a new typed detection method for norovirus detection and demonstrated that this method is sensitive and specific and can be used in the rapid detection for food borne virus.
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KEYWORD
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norovirus, molecular motor biosensor, detection, F0F1-ATPase
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